产品列表

k8凯发提供RUO和GMP两种级别的Anti-CD抗体，助力您从发现到临床阶段的研究。

• RUO级Anti-CD抗体

  产品编号	名称	数量	价格	操作
  A02199	Anti-Human CD3 Antibody (OKT3), mAb, Mouse		￥400.00 100 μg
  A02211	Anti-Human CD4 Antibody (F001), mAb, Mouse		￥400.00 100 μg
  A02212	Anti-Human CD8 Antibody (F002), mAb, Mouse		￥400.00 100 μg
  A02213	Anti-Human CD28 Antibody (F105), mAb, Mouse		￥400.00 100 μg

• GMP级Anti-CD抗体

  产品编号	名称	数量	价格	操作
  GMPA02199	GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse		- 100 μg 1 mg
  GMPA02213	GMP Anti-Human CD28 Antibody (F105), mAb, Mouse		- 100 μg 1 mg

• 新品预告

  产品编号	名称	订购
  GMPA02211	GMP Anti-Human CD4 Antibody (F001), mAb, Mouse	咨询
  GMPA02212	GMP Anti-Human CD8 Antibody (F002), mAb, Mouse	咨询

*没找到您需要的GMP级Anti-CD抗体？ 联系k8凯发并提供RUO级产品货号或CD分子名称。k8凯发的GMP蛋白平台可将RUO级目录产品快速转化为GMP级。

*k8凯发可根据您的要求提供抗体标记。包括 PE、FITC、iFluor 488、iFuor 555、iFuor 647 和Biotin。如需标记Anti-CD抗体，请联系k8凯发。

RUO 对比 GMP

• 质控放行标准

  项目	GMP*级抗体	RUO级抗体
  浓度	5.0 ± 0.5 mg/mL	1.0 ± 0.1 mg/mL
  外观	澄清、无色液体	澄清、无色液体
  纯度	SEC-HPLC：≥ 95%	SEC-HPLC：≥ 95%
  内毒水平	< 0.1 EU/mg	< 0.5 EU/μg
  活性	经验证	经验证
  鉴定	经质谱方法验证，序列与理论序列完全一致	/
  HCP残留	≤ 100.0 ng/mg	/
  HCD残留	≤ 10.0 pg/mg	/
  Protein A残留	≤ 10.0 ng/mg	/
  无菌	无菌	/

  *GMP 级是k8凯发用来描述在符合GMP规范的环境中生产的CD抗体的一个特定术语。该抗体生产遵循GMP的指导原则，具有严格的过程控制和完整的文件记录。k8凯发可提供文件记录，现场审计，及其它可帮助您的项目在特定区域进行申报的支持。详细了解k8凯发的 GMP的能力。

• GMP级Anti-CD抗体特色

  体外细胞培养可用性	GMP生产合规性	高稳定性和批间一致性
  无菌无动物源	可追溯文件记录	长期稳定性研究
  超低内毒水平（< 0.1 EU/mg）	符合GMP生产规范	加速稳定行研究
  HCD, HCP, Protein A残留控制	严格的质量控制和放行检测	冻融稳定性研究
  高纯度及高亲和力	可提供审计及法规相关支持	批次间质量一致

应用案例

• 抗人CD3和CD28抗体激活T细胞

  抗CD3和抗CD28抗体与 T 细胞表面表达的相应CD标志物结合，分别为T细胞激活提供第一和第二信号。共刺激的激活信号诱导T细胞快速增殖，在适应性免疫反应和免疫治疗中起着至关重要的作用。

  • 抗人CD3和CD28抗体激活T细胞快速增殖
    

    Figure 1. GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. GMPA02199) was used for primary T cell activation together with anti-Human CD28 antibody. The percentage of activated T cells were measured by flow cytometry. Data showed that GenScript GMP Anti-Human CD3 Antibody (OKT3), mAb, Mouse outperformed the competing products in activating primary T cells.

  • 抗人CD3和CD28抗体刺激报告细胞系信号响应
    

    Figure 2. CHO-K1/CD80 cells were plated 15 h before adding Jurkat/IL-2-Luc Promoter cells, then Jurkat/IL-2-Luc Promoter cells were stimulated with a serial of concentrations of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) for 5 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

    Figure 3. Jurkat/IL-2-Luc Promoter cells were co-stimulated with a serial of concentrations of Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) and 1 μg/mL of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) for 5 h. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway.

  • 原代T细胞激活鉴定和细胞因子分泌检测
    

    Figure 4. Primary T cells were stimulated with 5 μg/mL of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) (left), competing anti-Human CD3 antibody (middle), negative control (NC, right) as well as anti-Human CD28 antibody (all panels) for 72 h, respectively. CD25/CD69 expression was detected by flow cytometry to characterize cell activation.

    

    Figure 5. Primary T cell clumping was observed by a microscope after T cell activation (72 h) (right) using Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) and Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213). The inactivated T cells were presented on the left image.

    

    Figure 6. Interferon γ (IFN-γ) secretion by activated primary T cells using Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) and anti-Human CD28 antibody. Data showed that GenScript anti-Human CD3 antibody outperformed competing product.

    Figure 7. Interferon γ (IFN-γ) secretion by activated primary T cells using Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) and anti-Human CD3 antibody. Data showed that GenScript anti-Human CD28 antibody outperformed competing product.

• 抗人CD4和CD8抗体用于PBMC染色鉴定T细胞亚群

  抗CD抗体特异性识别细胞上表达的CD分子标志物，可用于进行细胞分群或功能鉴定。例如，CD4和CD8是PBMC染色中识别CD4+辅助T细胞和CD8+细胞毒性T细胞的常用标志物。

  

  Figure 8. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Human CD4 Antibody (F001), mAb, Mouse (Cat. No. A02211) (left), competing anti-human CD4 antibody (middle), mouse IgG1 negative control (NC, right) as well as with anti-Human CD3 [BV510] antibody (all panels). Flow cytometry was performed for further analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD.

  

  Figure 9. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Human CD8 Antibody (F002), mAb, Mouse (Cat. No. A02122) (left), competing anti-Human CD8 antibody (middle), mouse IgG1 negative control (right) as well as with anti-Human CD3 [BV510] antibody (all panels). Flow cytometry was performed for further analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and 7-AAD.

产品性能

• 高批间一致性
  

  Figure 10. Lot-to-lot consistency was tested using four batches of Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) on Jurkat/IL-2-Luc Promoter cells. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway. Data showed that GenScript Anti-Human CD3 Antibody (OKT3), mAb, Mouse (Cat. No. A02199) has a high lot-to-lot consistency.

  Figure 11. Lot-to-lot consistency was tested using four batches of Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) on Jurkat/IL-2-Luc Promoter cells. Receptor-mediated signaling induced luminescence via activation of the IL-2 pathway. Data showed that GenScript Anti-Human CD28 Antibody (F105), mAb, Mouse (Cat. No. A02213) has a high lot-to-lot consistency.

• 高稳定性
  

  Figure 12. Stability of GenScript GMP Anti-Human CD8 Antibody (F002), mAb, Mouse (Cat. No. GMPA02212) was tested. The data of long-term stability, accelerated stability, and freeze-thaw cycle stability indicated that this GMP antibody is very stable under various storage conditions.