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CHO-K1/FcRn Stable Cell Line

Figure 1: FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were stained with anti-FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz) and PE conjugated Goat anti-mouse IgG (minimal cross-reactivity) (Cat. No. 405307, BioLegend).

CHO-K1/FcRn Stable Cell Line

Recombinant CHO-K1 cells stably overexpress human neonatal Fc receptor (FcRn) and beta-2 microglobulin (B2M). The existence of B2M enables the stable expression of FcRn. The surface expression of FcRn is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, and antibody based biologics that binds with FcRn during biologics research and development.
M00603
￥85000

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产品概述
Product Description Recombinant CHO-K1 cells stably overexpress human neonatal Fc receptor (FcRn) and beta-2 microglobulin (B2M). The existence of B2M enables the stable expression of FcRn. The surface expression of FcRn is validated by FACS analysis. This stable cell line product is designed for measuring binding affinity and stability of Fc region of antibodies, Fc fusion proteins, and antibody based biologics that binds with FcRn during biologics research and development.
Applications Cell-based binding assay; use as immunogen for antibody development

产品简介
Expressed Gene Codon optimized from NM_004107 (for FcRn); codon optimized from NM_012673 (for B2M); no expressed tags
Target Protein NP_004098.1 (for FcRn); NP_036805.1 (for B2M)
Host Cell CHO-K1
Quantity 2 vials of frozen cells (>1×106 per vial in 1 ml)
Storage Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).

培养条件
Culture Properties Adherent
Freeze Medium 95% complete growth medium, 5% DMSO (Cat. No.D2650, Sigma)
Complete Growth Medium Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium Ham's F-12K (Kaighn's) (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 8 μg/ml Puromycin(Cat. No. A11138-03, Gibco), 400 μg/ml Hygromycin B (Cat. No. 10687010, Invitrogen)

技术背景
Gene Synonyms FCGRT (for FcRn); beta-2-Microglobulin (for B2M)
Background This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. 

图例
  • CHO-K1/FcRn Stable Cell Line
    • CHO-K1/FcRn Stable Cell Line

    Figure 1: FACS analysis of cell surface expression of FcRn on CHO-K1/FcRn cells.
    The CHO-K1/FcRn cells (blue) and the negative control CHO-K1 cells (red) were stained with anti-FcRn antibody (B-8) (Cat. No. sc-271745, Santa Cruz) and PE conjugated Goat anti-mouse IgG (minimal cross-reactivity) (Cat. No. 405307, BioLegend).


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