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DYKDDDDK Peptide

A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is co
RP10586CN
￥300

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产品概述
Synonyms DYKDDDDK tag;Flag-tag
Description A fusion tag, called DYKDDDDK and consisting of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK peptide.
Sequence
{ASP}{TYR}{LYS}{ASP}{ASP}{ASP}{ASP}{LYS}
Sequence Shortening DYKDDDDK
Molecular Formula C41H60N10O20
Molecular Weight 1012.97

产品属性
Purity > 95%
Form Lyophilized
Storage Store at -20°C.

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